n occultus  (ATCC)

Journal: Environmental Microbiology Reports

Article Title: Interplay of intracellular and trans‐cellular DNA methylation in natural archaeal consortia

doi: 10.1111/1758-2229.13258

Figure Lengend Snippet: Cryo‐ET analysis of the interaction between the host (SVX82) and DPANNs (SVXNc) in a coculture system. (A) Representative tomographic slice showing proteinaceous densities protruding out of the host cell (SVX82) surface (indicated by a yellow arrow). (B) Tomographic slice showing host‐DPANN interaction. Inset showing host‐DPANN interface bridged by a thin filament‐like density connecting the two cell types. (C) Tomographic slice showing a host‐DPANN interaction. Inset showing an enlarged view of the red‐boxed area. There is a dimple on the host surface and an inverted ‘V’ shaped protrusion on the DPANN surface (D) Representative tomographic slice indicating the enlarged view of the cytoplasmic bridges highlighted in red in the inset. The membrane boundaries of both cells look fuzzy where the cytoplasmic bridge is formed. (E) Tomographic slice showing extensive membrane blebbing from the DPANN SVXNc at the DPANN‐host interface. Blebs are highlighted in the red box. (F) 3D Segmentation analysis highlights key components: Host SVX82 S‐layer (dark purple), inner membrane (dark blue), outer membrane (light blue) ribosomes (yellow). Scale bars indicate (B–D) (100 nm).

Article Snippet: N. occultus SVXNc genome (Reva et al., ).

Techniques: Tomography, Membrane

Journal: Environmental Microbiology Reports

Article Title: Interplay of intracellular and trans‐cellular DNA methylation in natural archaeal consortia

doi: 10.1111/1758-2229.13258

Figure Lengend Snippet: Distribution of GDG c HC methylated sites in the Haloferax lucertense SVX82 genome when grown in different experiments: (I) pure (axenic) culture on d ‐xylose; (II) binary culture with the ectosymbiont Ca . N. occultus SVXNc on d ‐xylose; (III) binary culture with Halorabdus sp. SVX81 on xylan; (IV) trinary culture with Halorabdus sp. SVX81 and the ectosymbiont Ca . N. occultus SVXNc on xylan. Methylated and unmethylated loci are indicated by blue and green triangle labels, respectively. External and internal markings indicate respectively the methylation on the direct and the reverse complement DNA strands. Methylated residue in the motif label is depicted in lowercase in italics. The histogram curve shows fluctuations in GC content over a 5000 bp sliding window. Chromosomal and plasmid replicons are shown by solid brown and green arcs, respectively. Genetic regions masked from the analysis due to the unstable depth of sequencing are indicated. The numbers separated by a slash indicate respectively the numbers of methylated loci and the total number of GDGCHC motifs found in the H. lucertense SVX82 genome. Below the numbers is the percentage of methylated sites throughout the genome.

Article Snippet: N. occultus SVXNc genome (Reva et al., ).

Techniques: Methylation, Residue, Plasmid Preparation, Sequencing